PDBx:em_vitrificationType

<xsd:complexType name="em_vitrificationType">
  <xsd:annotation>
    <xsd:documentation xml:lang="en">Data items in the EM_VITRIFICATION category record details about the method and cryogen used in rapid freezing of the sample on the grid prior to its insertion in the electron microscope Example 1 - based on PDB entry 1DYL and laboratory records for the structure corresponding to PDB entry 1DYL <PDBx:em_vitrificationCategory> <PDBx:em_vitrification entry_id="1DYL" id="1"> <PDBx:citation_id>1</PDBx:citation_id> <PDBx:cryogen_name>ETHANE</PDBx:cryogen_name> <PDBx:details> SAMPLES WERE PREPARED AS THIN LAYERS OF VITREOUS ICE AND MAINTAINED AT NEAR LIQUID NITROGEN TEMPERATURE IN THE ELECTRON MICROSCOPE WITH A GATAN 626-0300 CRYOTRANSFER HOLDER.</PDBx:details> <PDBx:humidity>90</PDBx:humidity> <PDBx:instrument xsi:nil="true" /> <PDBx:method>PLUNGE VITRIFICATION</PDBx:method> <PDBx:sample_preparation_id>1</PDBx:sample_preparation_id> <PDBx:temp>95</PDBx:temp> <PDBx:time_resolved_state xsi:nil="true" /> </PDBx:em_vitrification> </PDBx:em_vitrificationCategory></xsd:documentation>
  </xsd:annotation>
  <xsd:sequence>
    <xsd:element name="em_vitrification" minOccurs="0" maxOccurs="unbounded">
      <xsd:complexType>
        <xsd:all>
          <xsd:element name="citation_id" minOccurs="0" maxOccurs="1" nillable="true" type="xsd:string">
            <xsd:annotation>
              <xsd:documentation xml:lang="en">This data item is a pointer to attribute id in category citation in the CITATION category.</xsd:documentation>
            </xsd:annotation>
          </xsd:element>
          <xsd:element name="cryogen_name" minOccurs="0" maxOccurs="1" nillable="true">
            <xsd:annotation>
              <xsd:documentation xml:lang="en">This is the name of the cryogen.</xsd:documentation>
            </xsd:annotation>
            <xsd:simpleType>
              <xsd:restriction base="xsd:string">
                <xsd:enumeration value="HELIUM"/>
                <xsd:enumeration value="NITROGEN"/>
                <xsd:enumeration value="PROPANE"/>
                <xsd:enumeration value="ETHANE"/>
                <xsd:enumeration value="ETHANE-PROPANE"/>
                <xsd:enumeration value="METHANE"/>
                <xsd:enumeration value="FREON 22"/>
                <xsd:enumeration value="FREON 12"/>
                <xsd:enumeration value="OTHER"/>
                <xsd:enumeration value="NONE"/>
              </xsd:restriction>
            </xsd:simpleType>
          </xsd:element>
          <xsd:element name="details" minOccurs="0" maxOccurs="1" nillable="true" type="xsd:string">
            <xsd:annotation>
              <xsd:documentation xml:lang="en">Any additional details relating to vitrification. Vitrification carried out in argon atmosphere.</xsd:documentation>
            </xsd:annotation>
          </xsd:element>
          <xsd:element name="humidity" minOccurs="0" maxOccurs="1" nillable="true" type="xsd:string">
            <xsd:annotation>
              <xsd:documentation xml:lang="en">The humidity (&#37;) in the vicinity of the vitrification process. 90</xsd:documentation>
            </xsd:annotation>
          </xsd:element>
          <xsd:element name="instrument" minOccurs="0" maxOccurs="1" nillable="true" type="xsd:string">
            <xsd:annotation>
              <xsd:documentation xml:lang="en">The type of instrument used in the vitrification process. Reichert plunger</xsd:documentation>
            </xsd:annotation>
          </xsd:element>
          <xsd:element name="method" minOccurs="0" maxOccurs="1" nillable="true" type="xsd:string">
            <xsd:annotation>
              <xsd:documentation xml:lang="en">The procedure for vitrification. blot for 2 seconds before plunging</xsd:documentation>
            </xsd:annotation>
          </xsd:element>
          <xsd:element name="sample_preparation_id" minOccurs="0" maxOccurs="1" nillable="true" type="xsd:string">
            <xsd:annotation>
              <xsd:documentation xml:lang="en">This data item is a pointer to attribute id in category em_sample_preparation in the EM_SAMPLE_PREPARATION category.</xsd:documentation>
            </xsd:annotation>
          </xsd:element>
          <xsd:element name="temp" minOccurs="0" maxOccurs="1" nillable="true">
            <xsd:annotation>
              <xsd:documentation xml:lang="en">The temperature (in degrees Kelvin) at which vitrification took place. 4.2</xsd:documentation>
            </xsd:annotation>
            <xsd:complexType>
              <xsd:simpleContent>
                <xsd:extension base="xsd:decimal">
                  <xsd:attribute fixed="kelvins" name="units" type="xsd:string" use="optional"/>
                </xsd:extension>
              </xsd:simpleContent>
            </xsd:complexType>
          </xsd:element>
          <xsd:element name="time_resolved_state" minOccurs="0" maxOccurs="1" nillable="true" type="xsd:string">
            <xsd:annotation>
              <xsd:documentation xml:lang="en">The length of time after an event effecting the sample that vitrification was induced and a description of the event. 30 msec after spraying with effector</xsd:documentation>
            </xsd:annotation>
          </xsd:element>
        </xsd:all>
        <xsd:attribute name="entry_id" use="required" type="xsd:string">
          <xsd:annotation>
            <xsd:documentation xml:lang="en">This data item is a pointer to attribute id in category entry in the ENTRY category.</xsd:documentation>
          </xsd:annotation>
        </xsd:attribute>
        <xsd:attribute name="id" use="required" type="xsd:string">
          <xsd:annotation>
            <xsd:documentation xml:lang="en">The value of attribute id in category em_vitrification must uniquely identify the vitrification procedure.</xsd:documentation>
          </xsd:annotation>
        </xsd:attribute>
      </xsd:complexType>
    </xsd:element>
  </xsd:sequence>
</xsd:complexType>